Abstract
The rate law governing the kinetics of a single enzyme mediated reaction may be derived relatively easily by hand given knowledge of the enzyme's mechanism. Such rate laws are typically non-linear in the concentrations of metabolites involved. When a number of enzymes interact the composite rate law for the complete system involves the simultaneous solution of the individual enzyme rate laws. We show how computer algebra can be used to solve this previously intractable problem, using the method of Gröbner Bases. We present an experimental example where kinetic parameters for individual enzymes are measured by making observations of a multi-enzyme system, and fitting these data to the rate law for the complete system.
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© 1991 Springer-Verlag
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Bennett, J.P., Davenport, J.H., Dewar, M.C., Fisher, D.L., Grinfeld, M., Sauro, H.M. (1991). Computer algebra approaches to enzyme kinetics. In: Jacob, G., Lamnabhi-Lagarrigue, F. (eds) Algebraic Computing in Control. Lecture Notes in Control and Information Sciences, vol 165. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0006927
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DOI: https://doi.org/10.1007/BFb0006927
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Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-54408-1
Online ISBN: 978-3-540-47603-0
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Keywords
- Malate Dehydrogenase
- Computer Algebra
- Computer Algebra System
- Steady State Assumption
- Factorise Coefficient
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