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Histogenic layer manipulation in chimeral ‘Thornless Evergreen’ trailing blackberry

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Summary

‘Thornless Evergreen’ blackberry (Rubus laciniatus Willd.) is a periclinal chimera in which the epidermis has mutated to a thornless phenotype while the internal portions of the plant possess the wild thorny genotype. Shoot tips were used to establish a source of experimental material. Nine hundred plants of ‘Thornless Evergreen’ were proliferated and rooted in vitro in an effort to locate a chimeral rearrangement and/or a pure thornless plant. When these tissue culture propagated plants were grown in the greenhouse, two predominant plant types were observed; about 53% of the propagules showed a normal vining growth habit while the other 47% of the plants were dwarfed due to shortened internodes. Adventitious shoots from isolated root segments of the normal plants were thorny, while those from many of the dwarfed plants had developed from epidermal cells of the parent. Such plants of epidermal origin are no longer chimeral but are of pure thornless genotype.

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Authors and Affiliations

  1. Department of Horticulture, University of Illinois, Urbana-Champaign, USA

    Ken McPheeters & Robert M. Skirvin

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  1. Ken McPheeters
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  2. Robert M. Skirvin
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Graduate Assistant and Associate Professor, respectively. Funds for this research were provided by the Illinois Expreiment Station. We thank Mel Chu and Alan Otterbacher for technical assistance.

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McPheeters, K., Skirvin, R.M. Histogenic layer manipulation in chimeral ‘Thornless Evergreen’ trailing blackberry. Euphytica 32, 351–360 (1983). https://doi.org/10.1007/BF00021443

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  • DOI: https://doi.org/10.1007/BF00021443

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