Abstract
Microinjection is a commonly used technique for DNA transformation in Caenorhabditis elegans. It is a powerful tool that links genetic and molecular analysis to phenotypic analysis. In this chapter we shall provide an overview of microinjection for germline transformation in worms. Our discussion will emphasize C. elegans reproductive biology, applications and protocols for carrying out microinjection in order to successfully obtain transgenic worms.
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Acknowledgments
We would like to thank members of the Singson lab for helpful comments and discussion. Research in the Singson lab has been supported by grants from the National Institutes of Health, the National Science Foundation, the Busch Biomedical Fund, and Johnson and Johnson Discovery Awards.
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Kadandale, P., Chatterjee, I., Singson, A. (2009). Germline Transformation of Caenorhabditis elegans by Injection. In: Carroll, D. (eds) Microinjection. Methods in Molecular Biology, vol 518. Humana Press. https://doi.org/10.1007/978-1-59745-202-1_10
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DOI: https://doi.org/10.1007/978-1-59745-202-1_10
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